Journal: Analytical chemistry
Article Title: Automated 3D-Printed Microfluidic Array for Rapid Nanomaterial-Enhanced Detection of Multiple Proteins
doi: 10.1021/acs.analchem.8b01198
Figure Lengend Snippet: Representations of 3D-printed immunoarray: (A) 3D-printed microfluidic array with chambers to hold sample, wash buffers, detection nanoparticles, and coreactant for ECL generation. Array is shown on left without detection chip and on right bonded to pyrolytic graphite sheet (PGS) microwell detection chip with reagent and sample chambers filled with dye solutions for visualization; (B) representative disposable PGS chip with heat transferred microwells printed using hydrophobic toner ink. Inset illustrates sandwich immunoassay on a single-wall carbon nanotube forest (SWCNT) in one microwell.
Article Snippet: Internal reagent and sample chambers have dimensions of 25 × 2 × 1 mm (L x W x H) and volumes of 48 ± 2 μ L. The detection channel has an open bottom that is converted into a closed detection chamber by bonding it with adhesive onto a paper-thin pyrolytic graphite microwell detection chip cut from pyrolytic graphite sheet (PGS, Panasonic).
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